See published article at https://www.frontiersin.org/articles/10.3389/fimmu.2021.728694/full
Finds and scores all framework mutations from input antibody file (csv format). Outputs normalized FR scores as a scatterplot. Verbose mode prints full pairwise alignment of each antibody. The output_mutations option creates a csv with all individual mutation scores. The output_csv option creates a csv with overall FR scores for each analyzed antibody.
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Input sequences are entered into FDA_Abs.csv
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Normalization constants are entered into normalization.csv
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Scatterplot output as svg file named FDA_Abs.svg
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Results from a sample run are included