plot_intercellular_flows ERROR：

[capuddddd]

When I use the below code to for visualization, the following error occurs.

fs.pl.plot_intercellular_flows(adata,
                                flow_network = flow_network,
                                flowsig_network_key = 'flowsig_network',
                                align_mode = 'horizontal',
                                width_scale = 2.0,
                                x_margin_offset = 0.3,
                                y_margin_offset = 0.0,
                                ax=ax)
Traceback (most recent call last):
  File "<stdin>", line 1, in <module>
  File "/public/home/99017/02users/07ca/00scRNA/Result_UCD1.3/Mammary_gland/final_rds/cellchat/flowsig/flowsig-main/flowsig/plotting/_plotting.py", line 126, in plot_intercellular_flows
    all_inflow_vars = [node for node in flow_vars if flow_var_info.loc[node]['Type'] == 'inflow']
  File "/public/home/99017/02users/07ca/00scRNA/Result_UCD1.3/Mammary_gland/final_rds/cellchat/flowsig/flowsig-main/flowsig/plotting/_plotting.py", line 126, in <listcomp>
    all_inflow_vars = [node for node in flow_vars if flow_var_info.loc[node]['Type'] == 'inflow']
  File "/public/home/99017/00envs/anaconda3/envs/flowsigenv/lib/python3.10/site-packages/pandas/core/generic.py", line 1577, in __nonzero__
    raise ValueError(
ValueError: The truth value of a Series is ambiguous. Use a.empty, a.bool(), a.item(), a.any() or a.all().

What could be the cause of this problem? @axelalmet
Thanks!

[axelalmet]

Hello,

This usually occurs when there's a variable that's labelled as both an 'inflow' and 'outflow' variable. Did you happen to run FlowSig using cell-cell communication inference that included cell-cell contact interactions, e.g., something like CDH1-CDH1?

This issue has come up enough that in the next version of FlowSig, I will probably have a case that accounts for this.

Best wishes,
Axel.

[capuddddd]

Thanks for your reply! i have checked my data, it does exist this kind of ligand and receptor, i will modify it and try it again